Biochemical Variability study in Genotype and Isolate of Alternaria Blight in Pigeonpea
Laxman Prasad Balai, R B Singh, Asha Sinha and S M Yadav
Keywords: Alternaria blight, Pigeonpea, genotype, resistant, enzyme activity, protein, sugar and amino acids
Biochemical constituent’s contents like, soluble proteins; enzyme activity, carbohydrate and amino acid are important in resistant to the crop plants and measured on four different days (70, 95, 120 &145 DAS) old plants. The contents are found of pigeonpea leaves of a set of twelve genotype, which were inoculated with representatives’ ten isolates. In the estimation of soluble protein, nitrate reductase, nitrite reductase activity, total soluble sugar, soluble reducing sugar, non reducing sugar and amino acids content have recorded in higher amounts in resistant genotype (IPA-7-2 & ICP-7220) followed by moderately resistant (DA-11 & ICP-13174) and moderately susceptible (ICP-4725 & ICP-11294), whereas lower amount susceptible (ICP-7182 & BSMR-736) genotype and highly susceptible genotype (MAL-24 & Bahar). The maximum soluble protein, enzyme activity, carbohydrate and amino acid content were found in resistant genotype at early stage of plants with minimum reduction whereas, lowest content was found in susceptible genotype old plants with highest reduction. It showed same trend in a-virulent isolate in which, lowest reduction soluble protein, enzyme activity, carbohydrate and amino acid content were found as compared to virulent (aggressive) isolate.
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