Volume 15, Issue 59, 2026
Research Article
Development and Validation of a UV–Visible Spectrophotometric Method of Valacyclovir Hydrochloride in Bulk Drug and Pharmaceutical Dosage Forms
Method of Valacyclovir Hydrochloride in Bulk Drug and Pharmaceutical Dosage Forms
Abhishek R. Bhardwaj, Abhishek Kumar, Parul Bisht, Kiran Dobhal*
Keywords: Antiviral, Acyclovir, Validation, Valacyclovir, UV-visible spectroscopy
DOI:10.37273/chesci.cs062057071
Abstract
The current study aimed to develop and validate simple and rapid UV-spectrophotometric method for the estimation of Valacyclovir Hydrochloride in bulk and pharmaceutical formulations as per ICH guidelines. A simple, fast, and accurate process for estimating valacyclovir hydrochloride in tablet and bulk form has been developed by UV spectroscopy in the current investigation. The solvent system employed in the proposed method were water, 0.25N hydrochloric acid, and 0.25N sodium hydroxide. Valacyclovir Hydrochloride found a good linear relationship over the concentration range 4-24 µg/ml in water, and 5 -30 µg/ml 0.25NHCl, and 0.25N NaOH respectively. The drug follows the linearity with a regression value of 0.9941, 0.9924, and 09935 in the respective solvents. The current investigation was also applied to tablet and % amount of drug which was estimated between 99.44% to 101.67% indicate the satisfaction level with the label claim. The recovery at 80%, 100%, and 120 level was measured in the range of 99.5 to 100%. Lessening of % RSD are revealing of the good accuracy and reproducibility of the method. It is clear from this study that the proposed methods for valacyclovir estimation in pharmaceutical preparation are quick, efficient, and specific. They may also be applied in routine analysis for the quantification of the drug in a dosage form. The current method was found simple and rapid tool for routine investigation of Valacyclovir Hydrochloride in the bulk and in the pharmaceutical dosage form.
References
[1] Dobhal K, Garg R, Singh A, Semwal A. Insight into the Natural Biomolecules (BMs): promising candidates as zika virus inhibitors. Infectious Disorders-Drug Targets Disorders). 2024 Nov 1;24(7):1-7.
[2] Zhdanov KV, Rudakova AV, Vasilev VV, Rogozina NV. Secondary prevention of congenital cytomegalovirus infection with valacyclovir: pharmacoeconomic aspects. Russian Family Doctor. 2025 Dec 17;29(4):53-9.
[3] Bhavya Sri K, Vaishnavi A, Pravallika J, Sumakanth M. RP-HPLC-UV method for estimation of antiviral drug (Valaciclovir) in pure, solid dosage form and spiked human plasma using MBTH reagent as per ICH guidelines. Accreditation and Quality Assurance. 2025 Aug;30(4):349-59.
[4] Chalikwar SS, Moravakar KK, Bhairav BA. Stability Indicating Method Development and Validation for Estimation of Valacyclovir in Pharmaceutical Preparation. Asian Journal of Pharmaceutical Analysis. 2024 Apr;14(2):53-9.
[5] Morgan EM, Lotfy HM, Fayez YM, Abdelkawy M. Comparative study of the selectivity power of colorimetric method over chromatographic methods for the analysis of valaciclovir hydrochloride. Journal of AOAC International. 2022 May 1;105(3):717-26.
[6] Bisht P, Kumar M, Saxena P, Kumar Gangwar A. To develop validated method of salicylic acid by UV-visible spectroscopy as impurity in pharmaceutical dosage form. World J Pharm Res. 2020 May 8;9(7):1981-9.
[7] Sahu SK, Patro SK, Giri RK. Method development and validation of valacyclovir in bulk and tablet dosage form by spectrophotometric and non-aqueous potentiometric method. World J Pharm Res. 2022 Jan 28;11(4):1240-56.
[8] Lasure AK, Ansari AF, Kalshetti MA. UV spectrophotometric analysis and validation of acyclovir in solid dosage form. International Journal of Current Pharmaceutical Research. 2020;12(2):100-3.
[9] Basavaiah K, Prameela HC. Simple spectrophotometric determination of acyclovir in bulk drug and formulations. Il Farmaco. 2002 May 22;57(6):443-9.
[10] Chundur J, Chadalawada PK, Babu GK, Puttagunta SB. Development and Validation of Analytical Procedures for the Simultaneous Estimation of Acyclovir and Zidovudine through UV and RP-HPLC Methods. World Journal of Pharmacy and Pharmaceutical Sciences. 2024 Aug 15;13(10):674-83.